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If generating v2 sample sheets for instruments aside from those mentioned, validate the ultimate sample sheet has the i5 index in the right orientation as explained over.
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Set D Index Adapters Make reference to Index 2 (i5) Orientation for more information on how to enter i5 bases about the sample sheet in ahead or reverse enhance orientation.
Because of this these computer software work on the idea that the i5 sequence is always within the forward orientation and can immediately produce the reverse enhance of your sequences presented for the appropriate instrument variety.
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When working with BaseSpace Sequence Hub PrepTab, i5 sequences have to be entered into these computer software in the ahead orientation in all situations and for all instrument styles.
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